Post-incubation Heating Significantly Improves Tyramide Signal Amplification

Tyramide Signal Amplification Strategies for Fluorescence Labeling

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

In our efforts to determine the patterns of expression and subcellular localization of Drosophila RNAs on a genome-wide basis, and in a variety of tissues, we have developed numerous modifications and improvements to our original fluorescent in situ hybridization (FISH) protocol. To facilitate throughput and cost effectiveness, all steps, from probe generation to signal detection, are performed...

Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification.

One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) protocol for whole-mount zebrafish embryos using tyramide signal amplification (...

Novel application of tyramide signal amplification (TSA): ultrastructural visualization of double-labeled immunofluorescent axonal profiles.

Fluorescent immunocytochemistry (FICC) allows multiple labeling approaches when enzyme-based techniques are difficult to combine, such as in double-labeling experiments targeting small-caliber axonal segments. Nevertheless, the conversion of FICC to a product visible at the electron microscopic (EM) level requires labor-intensive procedures, thus justifying the development of more user-friendly...

Automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification using antibodies from the same species

Background Immunohistochemical (IHC) detection of multiple antigens on the same tissue section represents a major unmet technological need in research and clinical diagnostics. While primary antibodies from different species have been used with differently labeled species-specific secondary antibodies, quite often the appropriate combination of antibodies is not available. More recently, primar...